BINA

We present a lightweight, flexible, transparent, and conductive bilayer composite of polyimide and single-layer graphene suspended on the centimeter scale with an unprecedentedly high aspect-ratio of 1e5. The coupling of the two components leads to mutual reinforcement and creates an ultra-strong membrane that supports 30,000 times its own weight. Upon electromechanical actuation, the membrane pushes a massive amount of air and generates high-quality acoustic sound. The energy efficiency is~ 10-100 times better than state-of-the-art electrodynamic speakers. The bilayer membrane’s combined properties of electrical conductivity, mechanical strength, optical transparency, thermal stability, and chemical resistance will promote applications in electronics, mechanics, and optics.

Objective. Restoration of central vision loss in patients with age-related macular degeneration (AMD) by implanting a retinal prosthesis is associated with an intriguing situation wherein the central prosthetic vision co-exists with natural normal vision. Of major interest are the interactions between the prosthetic and natural vision. Here we studied the effect of the light-adaptive state of the normal retina on the electrical visual evoked potentials (VEPs) arising from the retinal prosthesis. Approach. We recorded electrical VEP elicited by prosthetic retinal stimulation in wild-type rats implanted with a 1 mm photovoltaic subretinal array. Cortical responses were recorded following overnight dark adaption and compared to those recorded following bleaching of the retina by light (520 nm) at various intensities and durations. Main results. Compared to dark-adapted responses, bleaching induced a 2-fold decrease in the …

We study the magnetization dynamics of nanomagnetic beads with a diameter ranging from 80 to 250 nm in frequency and time domains using a high-resolution elliptical planar Hall effect (EPHE) sensor integrated with a fluidic channel. We find that the dynamics are well described with Cole–Cole and exponential decay models for frequency and time domains, respectively. We discuss the potential use of EPHE sensors in lab-on-a-chip applications.

S44. 00005: Imaging filamentary current flow near the metal-insulator transition in an oxide interface*

Chemical element mapping is an imaging tool that provides essential information on composite materials and it is crucial for a broad range of fields ranging from fundamental science to numerous applications. Methods that exploit x-ray fluorescence are very advantageous and are widely used, but require focusing of the input beam and raster scanning of the sample. Thus the methods are slow and exhibit limited resolution due to focusing challenges. We demonstrate a new focusing free x-ray fluorescence method based ghost imaging that overcomes those limitations. We combine our procedure with compressed sensing to reduce the measurement time and the exposure to radiation by more than 80%. Since our method does not require focusing, it opens the possibility for improving the resolution and image quality of chemical element maps with tabletop x-ray sources and for extending the applicability of x-ray fluorescence detection to new fields such as medical imaging and homeland security applications

Prophages are bacteriophages in the lysogenic state, where the viral genome is inserted within the bacterial chromosome. They contribute to strain genetic variability and can influence bacterial phenotypes. Prophages are highly abundant among the strains of the opportunistic pathogen Pseudomonas aeruginosa and were shown to confer specific traits that can promote strain pathogenicity. The main difficulty of studying those regions is the lack of a simple prophage-curing method for P. aeruginosa strains. In this study, we developed a novel, targeted-curing approach for prophages in P. aeruginosa. In the first step, we tagged the prophage for curing with an ampicillin resistance cassette (ampR) and further used this strain for the sacB counter-selection marker's temporal insertion into the prophage region. The sucrose counter-selection resulted in different variants when the prophage-cured mutant is the sole variant that lost the ampR cassette. Next, we validated the targeted-curing with local PCR amplification and Whole Genome Sequencing. The application of the strategy resulted in high efficiency both for curing the Pf4 prophage of the laboratory wild-type (WT) strain PAO1 and for PR2 prophage from the clinical, hard to genetically manipulate, 39016 strain. We believe this method can support the research and growing interest in prophage biology in P. aeruginosa as well as additional Gram-negative bacteria.

We report the experimental demonstration of efficient interaction of multi kilo electron Volt heralded x-ray photons with a beam splitter. The measured heralded photon rate at the outputs of the beam splitter is about 0.01 counts/s which is comparable to the rate in the absence of the beam splitter. We use this beam splitter together with photon number and photon energy resolving detectors to show directly that single x ray photons cannot split. Our experiment demonstrates the major advantage of x rays for quantum optics: the possibility to observe experimental results with high fidelity and with negligible background.

We investigate the overdamped Langevin motion for particles in a potential well that is asymptotically flat. When the potential well is deep as compared to the temperature, physical observables, like the mean square displacement, are essentially time-independent over a long time interval, the stagnation epoch. However, the standard Boltzmann–Gibbs (BG) distribution is non-normalizable, given that the usual partition function is divergent. For this regime, we have previously shown that a regularization of BG statistics allows for the prediction of the values of dynamical and thermodynamical observables in the non-normalizable quasi-equilibrium state. In this work, based on the eigenfunction expansion of the time-dependent solution of the associated Fokker–Planck equation with free boundary conditions, we obtain an approximate time-independent solution of the BG form, being valid for times that are long, but still short as compared to the exponentially large escape time. The escaped particles follow a general free-particle statistics, where the solution is an error function, which is shifted due to the initial struggle to overcome the potential well. With the eigenfunction solution of the Fokker–Planck equation in hand, we show the validity of the regularized BG statistics and how it perfectly describes the time-independent regime though the quasi-stationary state is non-normalizable.

In an effort to develop durable, corrosion-resistant catalyst support materials for polymer electrolyte fuel cells, modified polymer-assisted deposition method was used to synthesize tungsten carbide (WC, WC1–x), which was later used as a support material for Pt-based oxygen reduction reaction catalyst, as an alternative for the corrosion-susceptible, carbon supports. The Pt-deposited tungsten carbide’s corrosion-resistance, oxygen reduction reaction electrocatalysis, and durability were studied and compared to that of Pt/C. Bulk free carbon was found to be absent from the ceramic matrix which had particle size in the range of 2–25 nm. Tungsten carbide support appears to enhance the oxygen reduction activity on Pt, showing an increase in mass activity (nearly 2-fold at 0.85 V vs RHE) and specific activity (more than 7 times higher), alongside decrease in overpotential, in comparison to Pt/C. A significant increase in …

Mode locking in lasers is a collective effect, where due to a weak coupling a large number of frequency modes lock their phases to oscillate in unison, forming an ultrashort pulse in time. We demonstrate an analogous collective effect in coupled parametric oscillators, which we term “pairwise mode locking,” where many pairs of modes with twin frequencies (symmetric around the center carrier) oscillate simultaneously with a locked phase sum, while the phases of individual modes remain undefined. Thus, despite being broadband and multimode, the emission is not pulsed and lacks first-order coherence, while possessing a very high degree of second-order coherence. Our configuration comprises two coupled parametric oscillators within identical multimode cavities, where the coupling between the oscillators is modulated in time at the repetition rate of the cavity modes, with some analogy to active mode locking in …

Post-traumatic-stress-disorder (PTSD) is a stress-related condition that may develop after exposure to a severe trauma-event. One of the core brain areas that is considered to be a key regulatory region of PTSD is the amygdala. Specifically, the central amygdala (CeA) is involved in emotion processing and associative fear learning memory, two main circuits involved in PTSD. Long term dysregulation of trauma-related emotional processing may be caused by neuroadaptations that affect gene expression. The adenosine-(A) to-inosine (I) RNA editing machinery is a post-transcriptional process that converts a genomic encoded A to I and is critical for normal brain function and development. Such editing has the potential to increase the transcriptome diversity, and disruption of this process has been linked to various central nervous system disorders. Here, we employed a unique animal model to examine the possibility …

We demonstrate a new approach to supercontinuum generation and carrier-envelope-offset detection in dispersion-engineered nanophotonic waveguides based on saturated second-harmonic generation of femtosecond pulses. In contrast with traditional approaches based on self-phase modulation, this technique simultaneously broadens both harmonics by generating rapid amplitude modulations of the field envelopes. The generated supercontinuum produces coherent carrier-envelope-offset beatnotes in the overlap region that remain in phase across 100's of nanometers of bandwidth while requiring 10 picojoules of pulse energy.

We solve for the statistics of the first detection of a quantum system in a particular desired state, when the system is subject to a projective measurement at independent identically distributed random time intervals. We present formulas for the probability of detection in the n th attempt. We calculate as well the mean and mean square, both of the number of the first successful detection attempt and the time until first detection. We present explicit results for a particle initially localized at a site on a ring of size L, probed at some arbitrary given site, in the case when the detection intervals are distributed exponentially. We prove that, for all interval distributions and finite-dimensional Hamiltonians, the mean detection time is equal to the mean attempt number times the mean time interval between attempts. We further prove that for the return problem when the initial and target state are identical, the total detection probability is …

The interactions between antibodies, SARS-CoV-2 and immune cells contribute to the pathogenesis of COVID-19 and protective immunity. To understand the differences between antibody responses in mild versus severe cases of COVID-19, we analyzed the B cell responses in patients 1.5 months post SARS-CoV-2 infection. Severe, and not mild, infection correlated with high titers of IgG against Spike receptor binding domain (RBD) that were capable of ACE2:RBD inhibition. B cell receptor (BCR) sequencing revealed that VH3-53 was enriched during severe infection. Of the 22 antibodies cloned from two severe donors, six exhibited potent neutralization against authentic SARS-CoV-2, and inhibited syncytia formation. Using peptide libraries, competition ELISA and mutagenesis of RBD, we mapped the epitopes of the neutralizing antibodies (nAbs) to three different sites on the Spike. Finally, we used combinations of nAbs targeting different immune-sites to efficiently block SARS-CoV-2 infection. Analysis of 49 healthy BCR repertoires revealed that the nAbs germline VHJH precursors comprise up to 2.7% of all VHJHs. We demonstrate that severe COVID-19 is associated with unique BCR signatures and multi-clonal neutralizing responses that are relatively frequent in the population. Moreover, our data support the use of combination antibody therapy to prevent and treat COVID-19.

Quantum sensing exploits the strong sensitivity of quantum systems to measure small external signals. The nitrogen-vacancy (NV) center in diamond is one of the most promising platforms for real-world quantum sensing applications, predominantly used as a magnetometer. However, its magnetic field sensitivity vanishes when a bias magnetic field acts perpendicular to the NV axis. Here, we introduce a different sensing strategy assisted by the nitrogen nuclear spin that uses the entanglement between the electron and nuclear spins to restore the magnetic field sensitivity. This, in turn, allows us to detect small changes in the magnetic field angle relative to the NV axis. Furthermore, based on the same underlying principle, we show that the NV coupling strength to magnetic noise, and hence its coherence time, exhibits a strong asymmetric angle dependence. This allows us to uncover the directional properties of the …

In an effort to develop durable, corrosion-resistant catalyst support materials for polymer electrolyte fuel cells, modified polymer-assisted deposition method was used to synthesize tungsten carbide (WC, WC1–x), which was later used as a support material for Pt-based oxygen reduction reaction catalyst, as an alternative for the corrosion-susceptible, carbon supports. The Pt-deposited tungsten carbide’s corrosion-resistance, oxygen reduction reaction electrocatalysis, and durability were studied and compared to that of Pt/C. Bulk free carbon was found to be absent from the ceramic matrix which had particle size in the range of 2–25 nm. Tungsten carbide support appears to enhance the oxygen reduction activity on Pt, showing an increase in mass activity (nearly 2-fold at 0.85 V vs RHE) and specific activity (more than 7 times higher), alongside decrease in overpotential, in comparison to Pt/C. A significant increase in …

Insulin‐regulated glucose homeostasis is a critical and intricate physiological process, of which not all regulatory components have been deciphered. One of the key players in modulating glucose uptake by cells is the glucose transporter‐GLUT4. In this study, we aimed to explore the regulatory role of the trans‐Golgi‐associated protein‐TATA Element Modulatory Factor (TMF1) in the GLUT4 mediated, insulin‐directed glucose uptake. By establishing and using TMF1−/− myoblasts and mice, we examined the effect of TMF1 absence on the insulin driven functioning of GLUT4. We show that TMF1 is upregulated by insulin in myoblasts, and is essential for the formation of insulin responsive, glucose transporter GLUT4‐containing vesicles. Absence of TMF1 leads to the retention of GLUT4 in perinuclear compartments, and to severe impairment of insulin‐stimulated GLUT4 trafficking throughout the cytoplasm and to the …

Living in a group creates a complex and dynamic environment in which behavior of individuals is influenced by and affects the behavior of others. Although social interaction and group living are fundamental adaptations exhibited by many organisms, little is known about how prior social experience, internal states, and group composition shape behavior in groups. Here, we present an analytical framework for studying the interplay between social experience and group interaction in Drosophila melanogaster. We simplified the complexity of interactions in a group using a series of experiments in which we controlled the social experience and motivational states of individuals to compare behavioral patterns and social networks of groups under different conditions. We show that social enrichment promotes the formation of distinct group structure that is characterized by high network modularity, high inter-individual and …