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Jan 2023 • Power Ultrasonics, 431-454, 2023

Power ultrasound for the production of nanomaterials

A Gedanken, I Perelshtein, N Perkas

Sonochemistry in now well recognized as a technique for the fabrication of nanomaterials. This is reflected in the many review articles on sonochemistry and nanoparticles that have been published over the last few years. It is so happened that Suslick, one of the forefathers of this field, has lately written a very comprehensive review on this topic (Bang, 2010). In his review, Suslick has summarized the work published on sonochemistry and nanomaterials until 2010. The current review will try to scan the work done in this area until the end of 2012. The current review will concentrate first on explaining why nano? Namely, when, why, and what kind of nanomaterials are produced upon the collapse of the acoustic bubble?

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Jan 2023 • Proc. of SPIE Vol

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XX

Dror Fixler, Ewa M Goldys, Sebastian Wachsmann-Hogiu

PROCEEDINGS OF SPIE Page 1 PROCEEDINGS OF SPIE SPIEDigitalLibrary.org/conference-proceedings-of-spie Front Matter: Volume 12394 , "Front Matter: Volume 12394," Proc. SPIE 12394, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XX, 1239401 (2 May 2023); doi: 10.1117/12.2678752 Event: SPIE BiOS, 2023, San Francisco, California, United States Downloaded From: https://www.spiedigitallibrary.org/conference-proceedings-of-spie on 03 May 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use Page 2 PROGRESS IN BIOMEDICAL OPTICS AND IMAGING Vol. 24 No. 43 Volume 12394 Proceedings of SPIE, 1605-7422, V. 12394 SPIE is an international society advancing an interdisciplinary approach to the science and application of light. Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XX Dror Fixler Ewa M. Goldys Sebastian Wachsmann-…

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Jan 2023 • Proc. of SPIE Vol

Frontiers in Biological Detection: From Nanosensors to Systems XV

Amos Danielli, Benjamin L Miller, Sharon M Weiss

PROCEEDINGS OF SPIE Page 1 PROCEEDINGS OF SPIE SPIEDigitalLibrary.org/conference-proceedings-of-spie Front Matter: Volume 12397 , "Front Matter: Volume 12397," Proc. SPIE 12397, Frontiers in Biological Detection: From Nanosensors to Systems XV, 1239701 (2 May 2023); doi: 10.1117/12.2679008 Event: SPIE BiOS, 2023, San Francisco, California, United States Downloaded From: https://www.spiedigitallibrary.org/conference-proceedings-of-spie on 04 May 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use Page 2 PROGRESS IN BIOMEDICAL OPTICS AND IMAGING Vol. 24 No. 46 Volume 12397 Proceedings of SPIE, 1605-7422, V. 12397 SPIE is an international society advancing an interdisciplinary approach to the science and application of light. Frontiers in Biological Detection: From Nanosensors to Systems XV Amos Danielli Benjamin L. Miller Sharon M. Weiss Editors 29 …

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Jan 2023 • ACS Sustainable Chemistry & Engineering

Rechargeable Seawater Batteries Based on Polyimide Anodes

Amey Nimkar, Bar Gavriel, Gil Bergman, Meital Turgeman, Tianju Fan, Netanel Shpigel, Doron Aurbach

Being nearly unlimited natural resource containing mostly Na cations, the use of seawater as an electrolyte solution (aka seawater batteries) for electrochemical energy storage has received growing attention. To date, the vast majority of studies have focused on the use of seawater in Na-metal batteries protected by ion-conductive membranes hermetic to water. These systems, however, are complex and expensive, and suffer from a short cycling life. Here, we present alternative seawater batteries that utilize polyimide anodes. With its high capacity of more than 140 mAh/g, impressive rate capability, and excellent long-term stability (98% capacity retention after more than 9000 cycles), the prepared polyimide electrodes demonstrated to be promising candidate anodes for seawater electrochemical energy storage devices. Looking for a suitable cathode, we explored the use of nickel hexacyanoferrate (Ni-HCF) and …

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Jan 2023 • bioRxiv

Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Hadar Bootz-Maoz, Ariel Simon, Sara Del Mare-Roumani, Yifat Bennet, Danping Zheng, Sivan Amidror, Eran Elinav, Nissan Yissachar

The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex-vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

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Jan 2023 • iScience

Temporal Synchronization Elicits Enhancement of Binocular Vision Functions

Auria Eisen-Enosh, Nairouz Farah, Uri Polat, Yossi Mandel

Integration of information over the central nervous system is an important neural process that affects our ability to perceive and react to the environment. The visual system is required to continuously integrate information arriving from two different sources (the eyes) to create a coherent percept with high spatiotemporal precision. Although this neural integration of information is assumed to be critical for visual performance, it can be impaired under some pathological or developmental conditions. Here we took advantage of a unique developmental condition, amblyopia (“lazy eye”), which is characterized by an impaired temporal synchronization between the two eyes, to meticulously study the effect of synchronization on the integration of binocular visual information. We measured the eyes’ asynchrony and compensated for it (with millisecond temporal resolution) by providing time-shifted stimuli to the eyes. We found …

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Jan 2023 • Crystals

L-Glu Hierarchical Structure Crystallization Using Inorganic Ions

Michal Ejgenberg, Yitzhak Mastai

Hierarchical organic structures have gained vast attention in the past decade owing to their great potential in chemical and medical applications in industries such as the food and pharmaceutical industries. In this paper, the crystallization of L-glu hierarchical spheres using inorganic ions, namely calcium, barium and strontium cations, is described. The anti-solvent precipitation method is used for the spherical crystallization. The L-glu microspheres are characterized using various techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photo-electron microscopy (XPS) and polarized microscopy (POM). It is shown that without additives, L-glu crystallizes as flower-like structures, very different from the hierarchical spheres crystallized with the charged additives. Based on our results, we suggest a mechanism for the hierarchical sphere formation based on the crystallization and self-assembly of L-glu in emulsion droplets using charged additives.

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Jan 2023 • ACS Applied Materials & Interfaces

Dry synthesis of pure and ultrathin nanoporous metallic films

Hyunah Kwon, Hannah-Noa Barad, Alex Ricardo Silva Olaya, Mariana Alarcón-Correa, Kersten Hahn, Gunther Richter, Gunther Wittstock, Peer Fischer

Nanoporous metals possess unique properties attributed to their high surface area and interconnected nanoscale ligaments. They are mostly fabricated by wet synthetic methods that are not universal to various metals and not free from impurities due to solution-based etching processes. Here, we demonstrate that the plasma treatment of metal nanoparticles formed by physical vapor deposition is a general route to form such films with many metals including the non-noble ones. The resultant nanoporous metallic films are free of impurities and possess highly curved ligaments and nanopores. The metal films are ultrathin, yet remarkably robust and very well connected, and thus are highly promising for various applications such as transparent conducting electrodes.

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Jan 2023 • bioRxiv

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Ganesh Agam, Christian Gebhardt, Milana Popara, Rebecca Maechtel, Julian Folz, Ben Ambrose, Neharika Chamachi, Sang Yoon Chung, Timothy D Craggs, Marijn de Boer, Dina Grohmann, Taekjip Ha, Andreas Hartmann, Jelle Hendrix, Verena Hirschfeld, Christian G Huebner, Thorsten Hugel, Dominik Kammerer, Hyun Seo Kang, Achillefs Kapanidis, Georg Krainer, Kevin Kramm, Edward Lemke, Eitan Lerner, Emmanuel Margeat, Kirsten Martens, Jens Michaelis, Jaba Mitra, Gustavo G Moya Munoz, Robert Quast, Nicole Robb, Michael Sattler, Michael Schlierf, Jonathan Schneider, Tim Schroeder, Anna Sefer, Piau Siong Tan, Johann Thurn, Philip Tinnefeld, John van Noort, Shimon Weiss, Nicolas Wendler, Anders Barth, Claus AM Seidel, Don C Lamb, Thorben Cordes

Single-molecule FRET (smFRET) has become an established tool to study biomolecular structure and dynamics in vitro and in live cells. We performed a worldwide blind study involving 19 labs to assess the uncertainty of FRET experiments for proteins with respect to the measured FRET efficiency histograms, determination of distances, and the detection and quantification of structural dynamics. Using two protein systems that undergo distinct conformational changes, we obtained an uncertainty of the FRET efficiency of less than 0.06, corresponding to an interdye distance precision of less than 0.2 nm and accuracy of less than 0.5 nm. We further discuss the limits for detecting distance fluctuations with sensitivity down to less than 10% of the Foerster distance and provide guidelines on how to detect potential dye perturbations. The ability of smFRET experiments to simultaneously measure distances and avoid averaging of conformational dynamics slower than the fluorescence lifetime is unique for dynamic structural biology.

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Jan 2023 • Cellular and Molecular Gastroenterology and Hepatology

High-Resolution Genomic Profiling of Liver Cancer Links Etiology With Mutation and Epigenetic Signatures

Shira Perez, Anat Lavi-Itzkovitz, Moriah Gidoni, Tom Domovitz, Roba Dabour, Ishant Khurana, Ateret Davidovich, Ana Tobar, Alejandro Livoff, Evgeny Solomonov, Yaakov Maman, Assam El-Osta, Yishan Tsai, Ming-Lung Yu, Salomon M Stemmer, Izhak Haviv, Gur Yaari, Meital Gal-Tanamy

Background & AimsHepatocellular carcinoma (HCC) is a model of a diverse spectrum of cancers because it is induced by well-known etiologies, mainly hepatitis C virus (HCV) and hepatitis B virus. Here, we aimed to identify HCV-specific mutational signatures and explored the link between the HCV-related regional variation in mutations rates and HCV-induced alterations in genome-wide chromatin organization.MethodsTo identify an HCV-specific mutational signature in HCC, we performed high-resolution targeted sequencing to detect passenger mutations on 64 HCC samples from 3 etiology groups: hepatitis B virus, HCV, or other. To explore the link between the genomic signature and genome-wide chromatin organization we performed chromatin immunoprecipitation sequencing for the transcriptionally permissive H3K4Me3, H3K9Ac, and suppressive H3K9Me3 modifications after HCV infection.Results …

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Jan 2023 • Advanced Photonics Nexus

Toward augmenting tip-enhanced nanoscopy with optically resolved scanning probe tips

Jeremy Belhassen, Simcha Glass, Eti Teblum, George A Stanciu, Denis E Tranca, Zeev Zalevsky, Stefan G Stanciu, Avi Karsenty

A thorough understanding of biological species and emerging nanomaterials requires, among other efforts, their in-depth characterization through optical techniques capable of nanoresolution. Nanoscopy techniques based on tip-enhanced optical effects have gained tremendous interest over the past years, given their potential to obtain optical information with resolutions limited only by the size of a sharp probe interacting with focused light, irrespective of the illumination wavelength. Although their popularity and number of applications is rising, tip-enhanced nanoscopy (TEN) techniques still largely rely on probes that are not specifically developed for such applications, but for atomic force microscopy. This limits their potential in many regards, e.g., in terms of signal-to-noise ratio, attainable image quality, or extent of applications. We take the first steps toward next-generation TEN by demonstrating the fabrication …

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Jan 2023 • Ultrasonics Sonochemistry

Ultrasonic-assisted synthesis of lignin-capped Cu2O nanocomposite with antibiofilm properties

Moorthy Maruthapandi, Akanksha Gupta, Arumugam Saravanan, Gila Jacobi, Ehud Banin, John HT Luong, Aharon Gedanken

Under ultrasonication, cuprous oxide (Cu2O) microparticles (<5 µm) were fragmented into nanoparticles (NPs, ranging from 10 to 30 nm in diameter), and interacted strongly with alkali lignin (Mw = 10 kDa) to form a nanocomposite. The ultrasonic wave generates strong binding interaction between lignin and Cu2O. The L-Cu nanocomposite exhibited synergistic effects with enhanced antibiofilm activities against E. coli, multidrug-resistant (MDR) E. coli, S. aureus (SA), methicillin-resistant SA, and P. aeruginosa (PA). The lignin-Cu2O (L-Cu) nanocomposite also imparted notable eradication of such bacterial biofilms. Experimental evidence unraveled the destruction of bacterial cell walls by L-Cu, which interacted strongly with the bacterial membrane. After exposure to L-Cu, the bacterial cells lost the integrated structural morphology. The estimated MIC for biofilm inhibition for the five tested pathogens was 1 mg …

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Jan 2023 • Frontiers in Oncology

A predictive model for personalization of nanotechnology-based phototherapy in cancer treatment

Eli Varon, Gaddi Blumrosen, Orit Shefi

A major challenge in radiation oncology is predicting and optimizing a clinical response on a personalized manner. Recently, nanotechnology-based cancer treatments are being combined with photodynamic therapy (PDT) and photothermal therapy (PTT). Machine learning predictive models can be used to optimize the clinical setup configuration, such as: laser radiation intensity, treatment duration, and nanoparticles features. In this work we demonstrate a methodology to find the optimized treatment parameters for PDT and PTT by collecting data of in vitro cytotoxicity assay of PDT/PTT-induced cell death using a single nanocomplex. We examine three machine learning prediction models of regression, interpolation, and low degree analytical function to predict the laser radiation intensity and duration that maximize the treatment efficiency. To examine these prediction models accuracy, we built a dedicated dataset for PDT, PTT, and a combined treatment that is based on cell death measurements after light radiation treatment, divided to training and test sets. The preliminary results show that all models offer sufficient performance with death rate error of 0.09, 0.15, and 0.12 for the regression, interpolation, and analytical function fitting. Nevertheless, the analytical function due to its simple form has a clinical application advantage that can be used for further sensitivity analysis of the treatment parameters on the performance. In all, the results of this work form a baseline for a future machine learning base personal prediction model in combined nanotechnology-based phototherapy cancer treatment.

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Jan 2023 • Analysis & Sensing 3 (1), e202200053, 2023

Measurement of protein dynamics from site directed Cu (II) labeling

Kevin Singewald, Hannah Hunter, Timothy F Cunningham, Sharon Ruthstein, Sunil Saxena

This review describes the use of Electron Paramagnetic Resonance (EPR) to measure residue specific dynamics in proteins with a specific focus on Cu(II)‐based spin labels. First, we outline approaches used to measure protein motion by nitroxide‐based spin labels. Here, we describe conceptual details and outline challenges that limit the use of nitroxide spin labels to solvent‐exposed α‐helical sites. The bulk of this review showcases the use of newly developed Cu(II)‐based protein labels. In this approach, the strategic mutation of native residues on a protein to generate two neighboring Histidine residues (i.e., the dHis motif) is exploited to enable a rigid site‐selective binding of a Cu(II) complex. The chelation of the Cu(II) complex to dHis directly anchors the Cu(II) spin label to the protein backbone. The improvement in rigidity expands both the spin‐labeling toolkit as well as the resolution of many EPR …

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Jan 2023 • Crystals

L-Glu Hierarchical Structure Crystallization Using Inorganic Ions

Michal Ejgenberg, Yitzhak Mastai

Hierarchical organic structures have gained vast attention in the past decade owing to their great potential in chemical and medical applications in industries such as the food and pharmaceutical industries. In this paper, the crystallization of L-glu hierarchical spheres using inorganic ions, namely calcium, barium and strontium cations, is described. The anti-solvent precipitation method is used for the spherical crystallization. The L-glu microspheres are characterized using various techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photo-electron microscopy (XPS) and polarized microscopy (POM). It is shown that without additives, L-glu crystallizes as flower-like structures, very different from the hierarchical spheres crystallized with the charged additives. Based on our results, we suggest a mechanism for the hierarchical sphere formation based on the crystallization and self-assembly of L-glu in emulsion droplets using charged additives.

Show more

Jan 2023 • Analysis & Sensing 3 (1), e202200053, 2023

Measurement of protein dynamics from site directed Cu (II) labeling

Kevin Singewald, Hannah Hunter, Timothy F Cunningham, Sharon Ruthstein, Sunil Saxena

This review describes the use of Electron Paramagnetic Resonance (EPR) to measure residue specific dynamics in proteins with a specific focus on Cu(II)‐based spin labels. First, we outline approaches used to measure protein motion by nitroxide‐based spin labels. Here, we describe conceptual details and outline challenges that limit the use of nitroxide spin labels to solvent‐exposed α‐helical sites. The bulk of this review showcases the use of newly developed Cu(II)‐based protein labels. In this approach, the strategic mutation of native residues on a protein to generate two neighboring Histidine residues (i.e., the dHis motif) is exploited to enable a rigid site‐selective binding of a Cu(II) complex. The chelation of the Cu(II) complex to dHis directly anchors the Cu(II) spin label to the protein backbone. The improvement in rigidity expands both the spin‐labeling toolkit as well as the resolution of many EPR …

Show more

Jan 2023 • Power Ultrasonics, 431-454, 2023

Power ultrasound for the production of nanomaterials

A Gedanken, I Perelshtein, N Perkas

Sonochemistry in now well recognized as a technique for the fabrication of nanomaterials. This is reflected in the many review articles on sonochemistry and nanoparticles that have been published over the last few years. It is so happened that Suslick, one of the forefathers of this field, has lately written a very comprehensive review on this topic (Bang, 2010). In his review, Suslick has summarized the work published on sonochemistry and nanomaterials until 2010. The current review will try to scan the work done in this area until the end of 2012. The current review will concentrate first on explaining why nano? Namely, when, why, and what kind of nanomaterials are produced upon the collapse of the acoustic bubble?

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Jan 2023 • Polymer-Based Nanoscale Materials for Surface Coatings, 479-500, 2023

Superhydrophobic nanoscale materials for surface coatings

Sayan Ganguly, Shlomo Margel

Superhydrophobic surface preparation is developed by inspiration from nature. As it is a natural fact that lotus leaves are water repellant, thus researchers tried their best to develop superhydrophobic coatings by using several materials. The materials are categorized by inorganic, organic, and their synergistic hybrids. Polymeric coatings are more usable by scientists because of its tunable chemical features and their internal morphologies. This chapter will discuss in brief the coating materials and how polymer systems influenced the superhydrophobicity.

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Jan 2023 • ACS Applied Energy Materials

Performances of Co2+-Substituted NiMoO4 Nanorods in a Solid-State Hybrid Supercapacitor

Sengodan Prabhu, Moorthy Maruthapandi, Arulappan Durairaj, Srinivasan Arun Kumar, John HT Luong, Rajendran Ramesh, Aharon Gedanken

A hydrothermal method was conducted to synthesize Ni(1−α)Co(α)MoO4 (α = 0, 0.1, 0.3, and 0.5 M) nanorods, which were proven as excellent electrode materials in a hybrid supercapacitor. Their electrochemical properties were also dependent on the Ni/Co ratio as demonstrated by different electrochemical techniques. Ni0.5Co0.5MoO4 (α = 0.5 M) offered specific capacity (Qg) = 354 Cg–1@1 Ag–1, a remarkable specific capacity with a notable retention capacity of 92% after 8000 repeated cycles at 10 Ag–1. Ni0.5Co0.5MoO4 with a high surface area outperformed the mono-metallic (NiMoO4) and bimetallic (Ni0.9Co0.1MoO4 and Ni0.7Co0.3MoO4) nanostructures. The hybrid supercapacitor (Ni0.5Co0.5MoO4//activated carbon) delivered a maximum Qcell of 53 Cg–1 at 1 Ag–1 with an energy density of 16.2 Wh kg–1 and power density of 725 W kg–1.

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Jan 2023 • Polymer-Based Nanoscale Materials for Surface Coatings, 1-18, 2023

Introduction to coatings and surface preparation

Sayan Ganguly, Shlomo Margel

In this chapter, we would like to discuss polymer coating's know-how, which is a method of modifying surface qualities in order to satisfy operating requirements in a number of technological applications. In addition to adhesion and barrier capabilities, polymer coatings have also been used to improve scratch and abrasion resistance, solvent resistance, wettability, noncytotoxicity, and other features. For the manufacture of protective organic coatings a number of different techniques have been devised and used. A careful selection of polymer, coating process, and manufacturing conditions can result in high-performance coatings with improved attributes when applied correctly. Polymer coatings have recently been shown to be effective and widely used in a variety of applications, including solar cells, batteries, separation techniques, diodes, corrosion defense, packaging, and heathcare applications.

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Jan 2023 • Superconductor Science Technology

Characterization of YBa2Cu3O7-δ coplanar resonator for microwave kinetic inductance detectors

Ariel Roitman, A Shaulov, Y Yeshurun

We demonstrate an improved YBa 2 Cu 3 O 7-δ-based microwave kinetic inductance detector with a quality factor and noise equivalent power, $\sim {10^{-12}}{\mkern 1mu}{\text {W}}{\mkern 1mu}{\sqrt {{\text {Hz}}}^{-1}} $ at 10 K. Zero field cooled (ZFC) and field cooled (FC) measurements of the magnetic field dependence of the resonance characteristics, show substantially different behavior, indicating that both the screening currents and vortices play a role. The ZFC measurements exhibit a sharp decrease of the resonance frequency, , and at low fields, up to the full penetration field, revealing the dominant role of the screening currents. In contrast, the FC measurements exhibit a moderate decrease of and with field, revealing the role of vortices and reflecting the field dependence of the penetration depth in a d-wave superconductor.

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